Table 1.
PAMs for eight Cas9 and two Cas12a proteins
| Cas | Type | Species | Length | Consensus PAM | PDB (resolution) | PDB PAM |
|---|---|---|---|---|---|---|
| SpCas9 | II-A | Streptococcus pyogenes | 1368 | NGG | 4UN3 (2.59 Å) 5F9R (3.40 Å) |
TGG TGG |
| SaCas9 | II-A | Staphylococcus aureus | 1053 | NNGRRT | 5AXW (2.70 Å) 5CZZ (2.60 Å) |
TTGGGT TTGAAT |
| St1Cas9 | II-A | Streptococcus thermophilus | 1121 | NNRGAA | 6M0W (2.76 Å) | AAAGAA |
| FnCas9 | II-B | Francisella novicida | 1629 | NGG | 5B2O (1.70 Å) | TGG |
| Nme1Cas9 | II-C | Neisseria meningitidis | 1082 | NNNNGATT | 6JDV (3.10 Å) | ATATGATT |
| Nme2Cas9 | II-C | Neisseria meningitidis | 1082 | NNNNCC | 6JE3 (2.93 Å) | AGGCCC |
| CdCas9 | II-C | Corynebacterium diphtheriae | 1084 | NNRHHHY | 6JOO (2.90 Å) | GGGTAAT |
| AceCas9 | II-C | Acidothermus cellulolyticus | 1138 | NNNCC | 6WBR (2.91 Å) | ATACC |
| AsCas12a | V-A | Acidaminococcus sp. | 1307 | TTTV | 5B43 (2.80 Å) | TTTA |
| LbCas12a | V-A | Lachnospiraceae bacterium ND2006 | 1228 | TTTV | 5XUS (2.50 Å) | TTTA |