Table 3.

In vitro gas production (mL/g dry matter), dry matter (DM), and neutral detergent fiber (NDF) digestibility (%) at 24 and 48 h of single feedstuffs (N = 6) incubated or not (CON) with a Bacillus-based direct-fed microbial (DFM) in experiment 1^1,2

Item	Gas production			In vitro DM			In vitro NDF
	CON	DFM	SEM	CON	DFM	SEM	CON	DFM	SEM
24 h
Alfalfa hay	70.7b	78.4a	0.91	56.6b	58.3a	0.85	24.9	28.4	0.67
Beet pulp	105.0b	107.2a	1.22	57.4	58.2	0.63	23.5	26.0	0.73
Buffalo grass	73.0	75.1	1.59	41.8	43.7	1.77	30.9	33.2	1.85
Eragrostis hay	54.0b	58.8a	0.95	27.6	30.0	0.71	19.7	22.2	0.85
Oat hay	84.6	83.6	1.48	52.1	49.3	0.56	24.9	29.0	3.64
Smutsvinger grass	49.1b	55.8a	1.33	35.2	35.7	0.86	23.4	27.6	2.51
48 h
Alfalfa hay	98.3b	108.7a	3.47	69.4	73.0	2.04	37.5	46.8	2.78
Beet pulp	137.2b	151.6a	2.93	71.7b	78.3a	2.34	45.6b	64.0a	3.36
Buffalo grass	90.6	95.7	4.95	57.1	58.6	2.55	47.8	49.7	3.09
Eragrostis hay	70.7	72.1	1.89	40.6	38.1	1.06	30.1	28.2	0.97
Oat hay	106.6	109.4	4.99	59.7	62.1	1.64	44.6	52.0	5.92
Smutsvinger grass	67.3	70.4	1.30	43.4	44.6	0.44	29.2	30.4	0.53

¹ DFM, Bacillus-based direct-fed microbial incubated in the rumen fluid (B. licheniformis and B. subtilis; BOVACILLUS, Chr. Hansen A/S).

²Different letters on the same row denote differences at P < 0.05 level.