Table 1.

Body temperature, serum chemistry, and complete blood count response to an intravenous dose of 0.25 µg lipopolysaccharide/kg body weight in beef steers

Item	Pre-challenge1	Maximum	Minimum	SEM2	Max. hour	Min. hour	Δ%3	P-value4
Body temperature, °C
Rectal	38.8	39.6	38.7	0.271	2.5	−0.5	2	<0.01
Ocular	38.8	39.6	38.3	0.342	2.5	47.5	2	<0.01
Serum chemistry
Alanine aminotransferase, U/L	19.0	20.3	18.5	0.65	2	48	7	<0.01
Alkaline phosphatase, U/L	127.7	178.5	103.8	6.94	2	48	40	<0.01
Creatinine, mg/dL	0.77	0.82	0.76	0.027	12	48	6	0.22
Glucose, mg/dL	95.6	102.4	87.9	4.44	2	12	7	0.13
Total protein, g/dL	7.6	7.6	7.4	0.07	0	2	−3	0.01
Blood urea nitrogen, mg/dL	9.5	10.7	9.5	0.34	12	0	13	<0.01
Complete blood count
Red blood cells, M/µL	7.3	7.6	7.0	0.14	3	36	4	0.02
Hemoglobin, g/dL	9.8	10.2	9.4	0.16	3	24	4	<0.01
Hematocrit, %	28.1	29.3	27.2	0.57	3	36	4	0.02
Platelets, K/µL	506	516	381	24.8	−2	2	−25	<0.01
White blood cells, K/µL	11.4	12.6	2.9	0.55	36	1	−75	<0.01
Neutrophils, K/µL	4.2	4.3	0.4	0.41	−2	1	−90	<0.01
Lymphocytes, K/µL	5.1	7.0	2.0	0.43	12	1	−62	<0.01
Monocytes, K/µL	1.9	2.6	0.3	0.11	48	1	−84	<0.01
Eosinophils, K/µL	0.23	0.32	0.17	0.059	2	1	−29	<0.01
Basophils, K/µL	0.0029	0.0037	0.0000	0.00075	36	4	−100	<0.01
Neutrophils, %	35.3	36.0	14.4	3.21	−2	1	−59	<0.01
Lymphocytes, %	45.8	69.1	45.4	2.81	1	−2	51	<0.01
Monocytes, %	16.8	21.1	9.3	0.76	48	3	−45	<0.01
Eosinophils, %	2.0	7.8	1.5	0.63	2	48	292	<0.01
Basophils, %	0.025	0.028	0.000	0.0095	36	4	−100	0.34
Neutrophil:Lymphocyte	0.96	0.99	0.22	0.099	−2	1	−77	<0.01

¹Average of hours −2 and 0.

²Pooled standard error of the least squares mean.

³Percentage change between the peak value observed and the pre-challenge value.

⁴Observed significance level for the hour comparison.