Fig. 2.

Lentiviral vector transduction and NCX2 expression. A Lentiviral vector-transduced cells. U87-NC and U87-NCX2 were imaged under fluorescence microscope at × 400. B RT-PCR for NCX2 mRNA expression in U87MG. GAPDH was used as an internal reference. C Western blot analysis for NCX2. After transfection with NCX2, proteins from U87-NC and U87-NCX2 were collected and western blotting showed an increase in NCX2 protein. GAPDH was used as a loading control. *P < 0.01