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. 2022 Nov 5;61(3):979–994. doi: 10.1007/s10528-022-10274-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — HIF-1α knockdown and HIF-1α expression. A small interfering RNAs (siRNAs) cells. Control-siRNA and HIF-1α-siRNA were imaged under fluorescence microscope at × 400. B RT-PCR for HIF-1α mRNA expression in U87MG. GAPDH was used as an internal reference. C Western blot analysis for HIF-1α. After transfection with siRNAs, proteins from Control-siRNA and HIF-1α-siRNA were collected and western blotting showed a decrease in HIF-1α protein. GAPDH was used as a loading control. *P < 0.01