Fig. 4.

The morphologic and molecular characteristics of IL-17A–induced pyroptosis in CRC cells. A Representative microscopic images of CRC cells treated with IL-17A (100 ng/ml) for 72 h. Red arrows indicate the characteristic balloon in the cell membrane; B-C Expression of GSDME-N terminal by western blotting analysis after treated with IL-17A (100 ng/ml) for 72 h, data conforms to normal distribution, and tested by two independent samples t-test; D Representative scanning electron micrographs of CRC cells after treatment with IL-17A.Red arrows indicate formation of pyroptotic membrane pits and pores of varying size; E LDH release was detected in the supernates of CRC cells treated with IL-17A (100 ng/ml) for 72 h, data conforms to normal distribution, and tested by two independent samples t-test; F The release of IL-1β in the supernate was determined by ELISA, data conforms to normal distribution, and tested by two independent samples t-test; H-G Cell apoptosis rates were determined by using caspase-4 and propidium iodide co-dyeing staining assay after CRC cells treated with IL-17A (100 ng/ml) for 72 h, data conforms to normal distribution, and tested by two independent samples t-test; I Immunohistochemical results showing expression of IL-17A and IL-1β in colorectal tumour and normal tissues J Correlations between IL-17A and IL-1β in CRC