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. 2023 May 20;28:43. doi: 10.1186/s11658-023-00452-x

Table 2.

Summary of total number of labeled standard analytes which experienced matrix effect (suppression or enhancement) from plasma vs. serum

HILIC C18
MeOH MeOH:ACN ACN Hybrid-SPE/ACN Hybrid-SPE/MeOH MeOH MeOH:ACN ACN Hybrid-SPE/ACN Hybrid-SPE/MeOH
Plasma
 Suppressed (+ESI) 2 3 0 1 2 0 0 0 0 1
 Enhanced (+ESI) 1 0 3 0 0 4 2 3 4 2
 Total affected (+ESI) 3 3 3 1 2 4 2 3 0 3
 Total unaffected (+ESI) 0 0 0 1 1 0 1 0 0 1
 Suppressed (− ESI) 3 0 0 1 6 1 0 1 0 1
 Enhanced (− ESI) 1 2 5 3 0 0 2 1 3 1
 Total affected (− ESI) 4 2 5 4 6 1 2 2 3 2
 Total unaffected (− ESI) 2 3 0 2 0 3 2 0 0 2
Serum
 Suppressed (+ESI) 0 0 1 0 2 1 0 0 0 3
 Enhanced (+ESI) 1 1 1 3 0 1 4 4 1 1
 Total affected (+ESI) 1 1 2 3 2 2 4 4 1 4
 Total unaffected (+ESI) 2 1 1 0 0 2 0 0 0 0
 Suppressed (− ESI) 1 0 0 3 2 2 1 1 0 2
 Enhanced (− ESI) 5 6 6 3 0 1 2 2 3 0
 Total affected (− -ESI) 6 6 6 6 2 3 3 3 3 2
 Total unaffected (− ESI) 1 0 0 0 0 1 0 0 0 1

Table shows matrix effect of plasma vs. serum across five extraction methods (MeOH, MeOH:ACN, ACN, Hybrid-SPE/ACN, and Hybrid-SPE/MeOH) within four LC–MS analysis (+ ESI HILIC, − ESI HILIC, + ESI RP C18, and − ESI RP C18). An analyte is considered to have been affected if its matrix effect ratio is above 120% or suppressed if it is below 80%. (According to [6]). MeOH methanol, MeOH:ACN methanol combined with acetonitrile, ACN acetonitrile, SPE solid preparation extraction, ESI electrospray ionisation, RP reverse phase