Table 1. Sequence of primers used for genotyping Sdc1−/− and Gpc1−/− mice.
Primers Syndecan 1 forward and reverse and neomycin were used to distinguish knockout and wild type animals for the Sdc1−/− strain. The primers Gpc1 Mutant (forward and reverse) and Gpc1 wild type (forward and reverse) were used to distinguish knockout from wild type mice from the Gpc1−/− strain.
| Primer name | Forward | Reverse |
|---|---|---|
| Syndecan 1 (Sdc1) | CGC CGA AAC CTA CAG CCC TC | GCA TCG GCG AGT GGC GAG TC |
| Neomycin | CGAGACTAGTGAGACGTGCTACTTCC | |
| Glypican 1 Mutant | AGCCGGCTTTTGTTGTCTC | CACGAGTGTGCTAGGATAGGG |
| Glypican 1 Wild Type | CAGCGAAGTCCGCCAGAT | CAGACCTCCCGAGTGCTAGG |