TABLE 2.
Predicted Vsa protein characteristics
| Proteina | Repeat length (amino acids) | No. of repeatsb | Repeat pI | Protein pI | Protein mass (kDa) |
|---|---|---|---|---|---|
| VsaA | 17 | 32 | 8.0 | 8.5 | 80 |
| VsaA (CT) | 17 | 40 | 8.0 | 8.7 | 93 |
| VsaB | 13 | 39 | 4.0 | 4.3 | 80 |
| VsaC1 | 12 | 39 | 5.5 | 8.6 | 75 |
| VsaC2 | 12 | 7 | 2.8 | 4.8 | 40 |
| VsaC3 | 12 | 10 | 2.6 | 4.5 | 43 |
| VsaD1 | 11 | 11 | 4.3 | 5.0 | 45 |
| VsaD2 | 11 | 8 | 4.3 | 5.1 | 41 |
| VsaE1 | —c | — | — | 5.4 | 62 |
| VsaE2 | 14 | 11 | 6.1 | 8.8 | 41 |
| VsaE (CT) | — | — | — | 5.5 | 62 |
| VsaF | 11 | 8 | 4.0 | 4.9 | 40 |
| VsaG (CT) | 11 | 20 | 3.4 | 4.5 | 51 |
| VsaH (CT) | — | — | — | 9.1 | 31 |
a
Predicted proteins of strain CT are indicated by (CT). All other proteins are from KD735-15.
b
For the large proteins VsaA, VsaB, and VsaC1, the number of repeats was estimated by sizing pertinent restriction fragments and PCR products by agarose gel electrophoresis, as described previously (4). For the other proteins, the number of repeats was determined directly by sequence analysis of the corresponding vsa gene.
c
—, the protein lacks a tandem repeat domain.