TABLE 2.

Oligonucleotide primers used in this study

Primer no.a	Designation	Sequence (5′→3′)	Gene
1	rpoSfla-F1	TCCATCGTCAGCAAACACAGG	pdxK (BB768)
2	rpoSfla-F2	TGTTCGATGTTTGGACCTCC	gcp (BB769)
3	BB770-F1	TGTTTCCTCGTAAAGATTTAATAAGTTGTC	BB770
4	BB770-B1	GGATAAAAAAGCCAAAAGCAAACC	BB770
5	rpoS-F1	CCTATTTTTAAGTGGGGCATTTGC	BB770
6	rpoS-F3	GGTGCTTTTTTTGGGACTATTG	rpoS (BB771)
7	rpoS-B3	GCAGGACAAATACAAAGAGGC	rpoS (BB771)
8	rpoS-B1	AATTGGCACAGTTTTTGCATGG	5′-rpoS
9	rpoSfla-B2	TTAAGGTAAAAAGCAACAATTCATAC	BB773
10	rpoSfla-B1	ATCCAAAACTCAATTACAATATCTGAAGAG	5′-flgG
11	U178+BglII	ACCAGATCTTGTTGGTTTTAGCACTATA	5′-gyrBr
12	246R	CTCTTCATGAATATCGGTAGG	gyrBr
13	1580R	TTATAAAGAGGAGGCATGGC	gyrBr
14	1696F	CTTCAGAGATATAAAGGGCTTGGG	gyrBr
15	1905R+BclI	ACCTTGATCATTACACATCAAGATTAATTAC	gyrBr
16	Fl6	TTCAGGGTCTCAAGCGTCTTGGACT	flaB
17	Fl7	GCATTTTCAATTTTAGCAAGTGATG	flaB

^aThe numbers refer to the relative positions and orientations of primers in the rpoS flanking region and gyrB^r given in Fig. 1.