TABLE 2.

MLVA primers used to DNA type B. anthracis

Marker locus	Primers	Primer sequence	Dye labela	Tm (°C)
vrrA	vrrA-f1-fam	CAC AAC TAC CAC CGA TGG CAC A	Fam	71.0
	vrrA-r1	GCG CGT TTC GTT TGA TTC ATA C	None	69.7
vrrB1	vrrB1-f1-fam	ATA GGT GGT TTT CCG CAA GTT ATT C	Fam	70.0
	vrrB1-r1	GAT GAG TTT GAT AAA GAA TAG CCT GTG	None	69.0
vrrB2	vrrB2-f1-fam	CAC AGG CTA TTC TTT ATC AAA CTC ATC	Fam	72.0
	vrrB2-r1	CCC AAG GTG AAG ATT GTT GTT GA	None	68.8
vrrC1	vrrC1-f1	GAA GCA AGA AAG TGA TGT AGT GGA C	None	66.8
	vrrC1-r1-fam	CAT TTC CTC AAG TGC TAC AGG TTC	Fam	67.5
vrrC2	VrrC2-f1-hex	CCA GAA GAA GTG GAA CCT GTA GCA C	Hex	70.9
	vrrC2-r1	GTC TTT CCA TTA ATC GCG CTC TAT C	None	70.6
CG3	CG3-f1-ned	TGT CGT TTT ACT TCT CTC TCC AAT AC	Ned	66.2
	CG3-r1	AGT CAT TGT TCT GTA TAA AGG GCA T	None	66.2
pXO1-aat	pXO1-AAT-f3-fam	CAA TTT ATT AAC GAT CAG ATT AAG TTC A	Fam	66.3
	pXO1-AAT-r3	TCT AGA ATT AGT TGC TTC ATA ATG GC	None	66.7
pXO2-at	pXO2-AT-f1-hex	TCA TCC TCT TTT AAG TCT TGG GT	Hex	64.4
	pXO2-AT-r1	GTG TGA TGA ACT CCG ACG ACA	None	65.8

^aPE Biosystem's 5′ fluorescent phosphoramidite dyes: 6-Fam, Hex, and Ned.