TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| V. cholerae | ||
| N18 | Wild-type O1: Peru 1991 | J. M. Fournier (Institut Pasteur) |
| SV1 | N18 nhaA mutant | This work |
| SV2 | N18 nhaA′-lacZ nhaA mutant | This work |
| SV3 | N18 PnhaA′-lacZ | This work |
| E. coli | ||
| TG1 | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/supE Δ(hsdM-mcrB)5 (rK− mK− McrB−) thi Δ(lac-proAB) | Biolabs |
| DH5α λpir | recA1 gyrA (Nal) Δ(lacIZYA-argF) (φ80dlacΔ [lacZ]M15) pirRK6 | 27 |
| β2155 | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/thr-1004 pro thi strA hsdS Δ(lacZ)M15 ΔdapA::erm pir::RP4(::Kan from SM10) | D. Mazel (Institut Pasteur) and unpublished data |
| TA15 | melBLid nhaA+ ΔlacZY | 17 |
| NM81 | TA15 ΔnhaA1::kan+ thr-1 | 32 |
| HITΔAB− | HIT-1(nhaB mutant) ΔnhaA::Kanr | 51 |
| Plasmids | ||
| pBR322 | Ap Tc | 53 |
| pHG329 | Ap | 47 |
| pUC1318ΩKm1 | Ap Km; a pUC1318 derivative with a 1.5-kb ClaI fragment containing aphA-3 inserted at the SmaI site | P. Trieu-Cuot, unpublished data |
| pAT113/Sp | Sp Mob+ (IncP), oriR pACYC184, att Tn1545, lacZα+, MCS pUC19 | 8 |
| plac-1 | A pGP704 derivative with lacZ | 1 |
a
Ap, Tc, Nal, Km or Kan, and Sp, resistance to ampicillin, tetracycline, nalidixic acid, kanamycin and streptomycin, respectively.