TABLE 1.
Purification of 10 g (wet weight) of soluble cytochromes c from strain DSS12
| Experimental step | Total protein (mg) | Amt of heme c (μmol) | Content of cytochrome cA
|
Content of cytochrome cB
|
||||
|---|---|---|---|---|---|---|---|---|
| Amt of protein (mg) | Amt of heme c (μmol) | Concn of heme c (μmol/mg of protein) | Amt of protein (mg) | Amt of heme c (μmol) | Concn of heme c (μmol/mg of protein) | |||
| Soluble fraction | 377 | 173 | ||||||
| First anion exchange | 99.1 | 55.2 | ||||||
| Second anion exchange | 3.07 | 32.5 | 10.59 | 1.78 | 12.3 | 6.91 | ||
| Ammonium sulfate precipitation (80%) | 0.475 | 22.5 | 47.37 | 0.177 | 4.42 | 24.98 | ||
| Gel filtration (Superdex, 75 pg) | 0.223 | 16.2 | 72.65 | 0.085 | 2.61 | 30.70 | ||