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Detection of hybrid MBP-FimB protein by Western blotting. Polypeptides expressed from multicopy recombinant plasmids were detected by antibody against FimB (A) or MBP (B). The MBP-FimE fusion protein is included as a negative control in panel A (third lane from the left). The positions of native MBP (44 kDa) and MBP fusions (66 kDa) are indicated.
