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Comparison of wild-type and mutant R144Q binding to IRL-on in vitro. Shown is an electrophoretic mobility shift assay in which increasing concentrations of lysates containing MBP, wild-type MBP-FimB, and the MBP-FimB mutant R144Q were incubated with radiolabeled DNA amplified from the IRL component of fimS. Species corresponding to complexes with two bound recombinase protomers (DS) and a single bound protomer (SS) are shown. P is the unbound DNA probe (111 bp).
