TABLE 1.
PCR and DNA sequencing oligonucleotidesa
| OL 4 | 5′-GACAGAACAACGATTGCCAG-3′ |
| OL 20 | 5′-CTTTAAGGAGTGATTCGATTTC-3′ |
| R47AF | 5′-CATGGTTTCgcGGCGAGTGAAATTTGTCG-3′ |
| R47AR | 5′-CGACAAATTTCACTCGCCgcGAAACCATG-3′ |
| H141AF | 5′-GAGATTCATCCGgcCATGTTACGCCATTC-3′ |
| H141AR | 5′-GAATGGCGTAACATGgcCGGATGAATCTC-3′ |
| R144 | 5′-CACATGTTAcagCATTCGTG-3′ |
| Y176 | 5′-GTCTGGTtTACCGCCAGCAATG-3′ |
| H3Nsi | 5′-CAGGCATGCATGGCACTGGCCG-3′ |
| FimBR | 5′-AACAGGATCCTGGTATCTCAAC-3′ |
| FimBL | 5′-GGGAAAACATATGAAGAATAAGGCT-3′ |
a
The positions of nucleotide changes corresponding to amino acid substitutions are indicated in lowercase. The positions of restriction sites (NsiI, BamHI, and NdeI) within primers are indicated in boldface type.