TABLE 2.
Bacterial strains and plasmids
| Strain or plasmid | Relevant details | Reference or source |
|---|---|---|
| Strains | ||
| CJD808 | VL386; fimB::Kanr; phase locked on | 12 |
| CJD931 | VL386; fimB::Kanrihf::cml lrp::Tn10 | 12 |
| CJD957 | VL386; fimB::Kanr; phase locked off | 38 |
| CJD1353 | CJD808; recD::Tn10; 4-bp deletion in IRL | This work |
| DPB271 | recD::Tn10Δ16Δ17 | 5 |
| VL386 | Φ(fimA-lacZ)λpL(209) fimE::IS1 | 1 |
| VL386recD | VL386; recD::Tn10Δ16Δ17 | 38 |
| XL-1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZ M15 Tn10 (Tetr)] | Stratagene |
| Plasmids | ||
| pACYC184 | p15A replicon; Cmr Tetr | New England Biolabs |
| pLSB110 | pSLD203(R47A) | This work |
| pLSB111 | pSLD203(H141A) | This work |
| pLSB121 | pSGS501; phase on | This work |
| pLSB122 | pLSB121; 4-bp deletion in IRL | This work |
| pLSB201 | pSGS223(R144Q) | This work |
| pLSB202 | pSGS223(R47A) | This work |
| pLSB203 | pSGS223(H141A) | This work |
| pMalc-2 | Vector for creation of gene fusions to malE | New England Biolabs |
| pSGS223 | Plasmid encoding MBP-FimB protein fusion | This work |
| pSGS224 | pSGS223(Y176F) | This work |
| pSGS226 | pSLD203(Y176F) | This work |
| pSGS227 | pSLD203(R144Q) | This work |
| pSGS411 | fimE cloned in pUC18 | 38 |
| pSGS501 | fimB::KanrfimE::IS1 Φ(fimA-lacZ) in pACYC184 fimS; phase off | 38 |
| pSLD203 | fimB gene cloned in pUC18; Apr | 12 |
| pUC18 | ColE1 replicon; Apr | 40 |