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. 2023 May 4;9(5):720–732. doi: 10.1038/s41477-023-01405-0

Fig. 2. scRNA-seq reveals a leaf primordial rim cell type.

Fig. 2

a, Expression patterns of epidermal marker genes Ocl4 and Ltp2 compared with Wox3 genes. Arrows indicate rim cell cluster. b, Heatmap of more than 1,000 gene transcripts upregulated in cells from WT plants expressing one or more Wox3 genes compared with a randomly selected subset of 1,000 shoot apex cells. Two-sided Wilcoxon rank-sum test, Bonferroni-adjusted P < 0.05. c–j, RNA in situ hybridizations of the rim-upregulated genes Ga2ox3 (c,g), Ralf-like (d,h), Nga2-like (e,i), and Fpf1-like (f,j) in WT (c–f) and ns mutant (g–j) transverse shoot apex sections (n = 3). Scale bars, 100 µm. Max., maximum.