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. 2023 May 4;9(5):720–732. doi: 10.1038/s41477-023-01405-0

Fig. 5. Higher-order Wox3 mutants perturb mediolateral outgrowth of the leaf primordium.

Fig. 5

a–e, Whole shoot phenotypes of WT (a), ns1;ns2 (b), ns1;ns2;wox3a (c), ns1;ns2;wox3a;Wox3b+/wox3b (d), and ns1;ns2;wox3a;wox3b 4-week-old maize seedlings (e). Scale bars, 10 cm. f–k, Epifluorescence microscopy of the P4–P5 disc of insertion (f–h) and lateral views of P1–P2 (i–k) in (f,i) WT, (g,j) ns1;ns2, and (h,k) ns1;ns2;wox3a. l–n, RNA in situ hybridization of Cuc2 transcripts in transverse sections through the shoot apices of WT (l), ns double mutant (m) and ns1;ns2;wox3a triple mutant (n) seedling shoot apices at the P4 stage. White dotted lines outline the P4-staged primordium. Scale bars, 100 μm. o, Illustrations of indicated genotypes and their effects on leaf mediolateral domain development. p–r, RNA in situ hybridization of Ns1/2 (p) and Wox3a/b (q) in WT and of Ns1/2 (r) in the wox3a;wox3b mutant background in transverse sections of early-staged, initiating P1 leaf primordia. Scale bars, 100 μm. s,t, RNA in situ hybridization of Ns1/2 (s) and Wox3a/b (t) transcripts in transverse sections through the upper region of WT P2 leaf primordia. Scale bars, 100 μm. u,v, RNA in situ hybridization of Ns1/2 transcripts in two serial transverse sections slightly below (u) and at the tip (v) of the P2 primordium in a wox3a;wox3b mutant. Arrowheads indicate expected position of expression foci. Scale bars, 100 μm. w, Proposed expression patterns of Ns1/2 and Wox3a/b along the proximodistal axis of the leaf primordial rim. For all micrograph data presented in this figure, n = 3.