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. 2023 May 4;9(5):720–732. doi: 10.1038/s41477-023-01405-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Filtering of cell barcodes based on minimum genes per cell detected (3,000) and maximum Unique Molecular Identifiers (UMIs, that is transcripts) detected per cell (100,000). b, Further filtering of cell barcodes based on percentage of transcripts mapping to mitochondrial genes (1%). c, Summary statistics for the four libraries. d, UMAP plots with cells labelled by genotype.