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. Author manuscript; available in PMC: 2024 Jun 1.
Published in final edited form as: Gastroenterology. 2023 Mar 4;164(7):1137–1151.e15. doi: 10.1053/j.gastro.2023.02.030

Figure 3. Influence of stiffness on YAP expression and sequestration in crypt-like and villus-like regions.

Figure 3.

On the stiff gel, nuclear localization of YAP was shown on both crypt (A) and villus (B) regions. In villus regions, cyto-YAP expression was absent on the soft matrix and highly expressed on the medium matrix (B). (n=3). Scale bar in (A) and (B), 10 μm. (C) The non-phosphorylated nuclear (nuc-) YAP was increased by stiffening and showed clear nuclear localization on the stiff matrix (n=5). Scale bar, 25 μm. (D) The Ser 127 phosphorylated cytoplasmic (cyto-) YAP was decreased by stiffening in the crypt-like regions, but increased in the villus-like regions (n=5). Scale bar, 25 μm.