Figure 2. Sca2 comparison with formins.
Sca2 from our cryo-EM analysis compared with crystal structures of various formins. (A) Top- Sca2 model, with NRD residues 34-400 in red, residues 401-670 in yellow, and CRD residues 1090-1355 shown in green. Bottom- Sca2 model showing only residues 34-670, with CRD residues hidden. (B-E) Crystal structures of different formins. FH2 monomers are colored red or yellow for a straightforward comparison, and in (B-C) actin is colored in blue (B) Top- FMNL3 bound to actin (PDB 4EAH [37]). Bottom- Same as top, actins hidden. (C) Top- Bni1p bound to actin (PDB 1Y64 [35]). Bottom- Same as top, actins hidden. (D) DAAM1 (PDB 2Z6E [36]). (E) Bni1p (PDB 1UX5 [34]).