TABLE 1.
E. coli K-12 strains, bacteriophages, and plasmids used in this studya
| Strain, phage, or plasmid | Relevant characteristic(s) or genotype | Reference or source |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC ptsF25 rbsR | 42 |
| JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ traD36 proAB+ lacIqlacZΔM15 | Promega |
| JRG1728 | Δfnr::Tn9(Cmr) | 43 |
| DHB4 | araD139 Δ(ara-leu)7697 ΔlacX74 ΔphoA(PvuII) phoRΔmalF3 galE galK thi rpsL/F′ lacIqpro | 6 |
| JC7623 | recB21 recC22 sbcB15 sbcC201 | 36 |
| ECL5000 | JC7623 but ΔarcB::Tetr | This study |
| ECL5001 | MC4100 but Φ(cydA′-lacZ) | This study |
| ECL5002 | MC4100 but Φ(lldP′-lacZ) | This study |
| ECL5003 | MC4100 but Δfnr::Tn9(Cmr) Φ(cydA′-lacZ) | This study |
| ECL5004 | ΔarcB::Tetr Φ(cydA′-lacZ) Δfnr::Tn9(Cmr) | This study |
| ECL5005 | ΔarcB::Kanr Φ(cydA′-lacZ) Δfnr::Tn9(Cmr) | This study |
| ECL5006 | arcB+::Kanr Φ(cydA′-lacZ) Δfnr::Tn9(Cmr) | This study |
| ECL5007 | arcBH47Q::Kanr Φ(cydA′-lacZ) Δfnr::Tn9(Cmr) | This study |
| ECL5008 | arcBH47R::Kanr Φ(cydA′-lacZ) Δfnr::Tn9(Cmr) | This study |
| ECL5009 | Φ(arcB1-22-malF17-35-arcB42-778)::Kanr Φ(cydA′-lacZ) Δfnr::Tn9(Cmr) | This study |
| ECL5010 | Φ(arcB1-57-malF40-58-arcB78-778)::Kanr Φ(cydA′-lacZ) Δfnr::Tn9(Cmr) | This study |
| ECL5011 | Φ(arcB1-22-malF17-39-arcB58-778)::Kanr Φ(cydA′-lacZ) Δfnr::Tn9(Cmr) | This study |
| ECL5012 | ΔarcB::Tetr Φ(lldP′-lacZ) | This study |
| ECL5013 | ΔarcB::Kanr Φ(lldP′-lacZ) | This study |
| ECL5014 | arcB+::Kanr Φ(lldP′-lacZ) | This study |
| ECL5015 | arcBH47Q::Kanr Φ(lldP′-lacZ) | This study |
| ECL5016 | arcBH47R::Kanr Φ(lldP′-lacZ) | This study |
| ECL5017 | Φ(arcB1-22-malF17-35-arcB42-778)::Kanr Φ(lldP′-lacZ) | This study |
| ECL5018 | Φ(arcB1-57-malF40-58-arcB78-778)::Kanr Φ(lldP′-lacZ) | This study |
| ECL5019 | Φ(arcB1-22-malF17-39-arcB58-778)::Kanr Φ(lldP′-lacZ) | This study |
| Phages | ||
| λRS45 | ′bla ′lacZ lacY+ | 42 |
| λLPZ1 | Φ(lldP′-lacZ+) lacY+ | This study |
| λCAZ1 | Φ(cydA′-lacZ+) lacY+ | This study |
| P1vir | Laboratory stock | |
| Plasmids | ||
| pUC18 | Cloning vector | Stratagene |
| pBluescript SK(−) | Cloning vector | Strategene |
| pBluescript KS II(+) | Cloning vector | Stratagene |
| pNK81 | Tetr | 50 |
| pUC4-KIXX | Kanr | 3 |
| pBB25 | arcB+ | 23 |
| pDHB5747 | ′phoA | D. Boyd |
| pDHB32 | malF+ | 6 |
| pBP22 | Φ(arcB1-22-′phoA) | This study |
| pBP41 | Φ(arcB1-41-′phoA) | This study |
| pBP57 | Φ(arcB1-57-′phoA) | This study |
| pBP102 | Φ(arcB1-102-′phoA) | This study |
| pLCT2 | lldPRD+ | 9 |
| pLLD2 | lldPR+ | This study |
| pRS415 | lacZ+ lacY+ bla+ | 42 |
| pLPZ1 | Φ(lldP′-lacZ) | This study |
| pBTKScyd1 | cydA′ | 31 |
| pRS528 | lacZ+ lacY+ bla+ | 42 |
| pCAZ1 | Φ(cydA′-lacZ) | This study |
| pDB3 | ΔarcB::Tetr | This study |
| pIB3 | ΔarcB::Kanr | This study |
| pQE30ArcB78-778 | His6-ArcB78-778 | 14 |
| pABW | arcB+ in pBluescript KS II(+) | This study |
| pABS | arcB78-778 in pBluescript KS II(+) | This study |
| pIBW | arcB+::Kanr in pIB3 | This study |
| pIBHQ | arcBH47Q::Kanr in pIB3 | This study |
| pIBHR | arcBH47R::Kanr in pIB3 | This study |
| pIBM1 | Φ(arcB1-22-malF17-35-arcB42-778)::Kanr in pIB3 | This study |
| pIBM2 | Φ(arcB1-57-malF40-58-arcB78-778)::Kanr in pIB3 | This study |
| pIBM3 | Φ(arcB1-22-malF17-39-arcB58-778)::Kanr in pIB3 | This study |
a
Luria-Bertani broth and agar (17 g/liter) were used for routine growth. When used, ampicillin, tetracycline, kanamycin, and chloramphenicol were provided at final concentrations of 50, 12, 40, and 20 μg/ml, respectively.