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. 2023 May 12;92:104596. doi: 10.1016/j.ebiom.2023.104596

Fig. 2.

Fig. 2

Sporadic ChRCCs and sporadic oncocytomas formed two distinct subclusters characterized by L1CAM and FOXI1 expressions. (a) Scatter plot of principal component analysis (PCA) for Fig. 1b is shown. gL1CAM: group of sporadic ChRCCs and sporadic oncocytoma with predominant expression of L1CAM. gFOXI1: group of sporadic ChRCCs and sporadic oncocytomas with predominant expression of FOXI1. (b) Heatmap shows top fifty genes differentially expressed in Group1 (gL1CAM) and Group2 (gFOXI1). Blue triangles indicate L1CAM, FOXI1 and its downstream gene, ATP6V0D2. (c) Uniform Manifold Approximation and Projection (UMAP) shows cell clusters of BHD-associated ChRCC and BHD-associated HOCT. BHD-associated HOCT is comprised of two distinct cell clusters characterized by L1CAM expression (cL1CAM) or by FOXI1 expression (cFOXI1) (upper panel). Dot plot shows differentially expressing patterns of Notch genes and downstream genes in BHD-associated renal tumours (lower panel). (d) Scatter plots show an inverse correlation of FOXI1 and L1CAM expression levels (upper panel). gL1CAM: group of sporadic ChRCCs with predominant expression of L1CAM. gFOXI1: group of sporadic ChRCCs with predominant expression of FOXI1. gNeutral; group of sporadic ChRCCs which were clustered into neither gL1CAM nor gFOXI1. Pearson's correlation coefficient and p-value are shown. (e) Scatter plot shows an inverse correlation of FOXI1 and L1CAM expressions (lower panel). Pearson's correlation coefficient and p-value are shown. (f) Immunohistochemistry (IHC) of L1CAM protein (upper panel) or FOXI1 protein (lower panel) in sporadic ChRCCs. Scale bars represent 50 μm.