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. 2023 May 8;14:1158758. doi: 10.3389/fimmu.2023.1158758

Figure 4.

Figure 4

Axl skewed macrophage polarization toward M1 via STAT1/HIF-1α activation. A. STAT1 phosphorylation and HIF-1α activation measured by western blot 6 h after incubation with R428 or rmGas6 (A, B). Verification of the efficiency of STAT1 knockdown at the mRNA (C) and protein levels (D). The effects of STAT1 knockdown on IL-1β, Nos2, and MMP9 secretion were measured by qRT-PCR (E—G) and western blotting in rmGas6-treated BMDMs (H, I). ns, not significant, *P<0.05, **P< 0.01, ***P< 0.001, ****P< 0.0001.