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. 2023 May 8;14:1111680. doi: 10.3389/fpls.2023.1111680

Figure 3.

Figure 3

Gene editing on KTI1 has been completed for all seeds of T0 generation while it has been completed on KTI3 for some seeds of T0 generation. From each transgenic line (#2, #5, #11 and #17), four seeds of T0 generation were selected randomly for genotyping. (A) The gr of mutant kti1 in T0 seeds and T1 plant (#5-26) leaf, where the wild type KTI1 in WM82 was the control. (B) Gel electrophoresis of kti1 PCR products showed 16 seeds from line #2, #5, #11, and #17 had the same mutant on kti1, in which 66 nucleotides are lost between two sgRNAs. (C) Sanger sequencing result displayed the identical mutant kti1. (D) The alignment of mutant kti3 in T0 seeds (#2-3, #5-4, #5-26, #11-2 and #11-4) and T1 plant (#5-26) leaf, where the wild type KTI1 in WM82 was the control. (E–G) showed the sanger sequencing results of kti3 mutant in #2-3, #5-4, #11-2, #11-4, and #5-26.