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KTI content declined dramatically in gene-edited seeds. KTI content was measured in 4 double mutated seeds (#2-3, #5-4, #11-2, and #11-4) and 4 seeds with a single mutation on KTI1 (#2-1, #5-1, #11-1, and #17-1), where the KTI content in WM82 seed served as the control. Experiments were conducted with three technical replicates and showed comparable results, shown as mean ± s.e. Different letters indicate significant differences.
