. 2000 May;182(10):2978–2981. doi: 10.1128/jb.182.10.2978-2981.2000

TABLE 1.

Accumulation of PHA in recombinant E. coli strains carrying pET200 and pBTC2^a

Strain	Plasmid(s)	Gene (relevant markers)	PHA content (% cell dry wt)	Composition (mol%)^b			M_w
Strain	Plasmid(s)	Gene (relevant markers)	PHA content (% cell dry wt)	3HHx	3HO	3HD	M_w
MF4100	pBTC2	phaC⁺	—^c	—			—
	pBTC2, pET200	phaC⁺, fabG⁺	2.8	10	90	0	48,200

RS3338	pBTC2	phaC⁺	—	—			—
	pBTC2, pET200	phaC⁺, fabG⁺	3.1	9	91	0	51,100
JMU193	pBTC2	phaC⁺	4.6	11	76	13	56,400
	pBTC2, pET200	phaC⁺, fabG⁺	5.2	10	77	13	52,700

JMU194	pBTC2	phaC⁺	14.2	20	67	13	68,300
	pBTC2, pET200	phaC⁺, fabG⁺	20.6	23	65	12	67,000

E. coli recombinants were cultivated as described in the text. Determination of the PHA content and the PHA molecular weight was carried out as described previously (8, 11). Average data of three independent experiments are given.

3HHx, 3-hydroxyhexanoate; 3HO, 3-hydroxyoctanoate; 3HD, 3-hydroxydecanoate.

—, <0.1%.