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Affinity analysis of JEV TR RNA cyclization. (A) MST raw data traces for JEV 3′ TR + 5′ TR. Blue line represents the ‘cold’ time and the red line represent the ‘hot’ region, and the difference between the two is used to calculate the ΔFnorm. (B) Microscale thermophoresis measurements of different combinations of JEV TRs representing concentration versus fraction bound. Measurement ran on ‘high’ MST power.
