Figure 2.

Coordination of Cd²⁺ by the CBL-loop. (A) The 2F_o–F_c electron density maps (contour level, 1.5σ) of Cd²⁺ and the coordinating nucleotides. (B) The detailed coordination of the Cd²⁺. (C) Comparison of the CD spectra of the native DNA1 and the G9A, C12T and G16A variants. (D) Comparison of Cd²⁺ binding by the native DNA1 and the G9A, C12T and G16A variants by ITC assays. In panels (A) and (B), Cd²⁺ ion and the Cd²⁺-coordinating water molecules are shown as spheres in black and red, respectively.