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. 2023 Mar 17;51(9):4555–4571. doi: 10.1093/nar/gkad195

Figure 2.

Figure 2.

(A) Overlay of 1D-1H-SOFAST NMR spectra focused on the imino resonances of s2m-SARS-CoV-2 (50 μM) at increasing ratios of CTD (25 mM Na Phos, 50 mM NaCl pH 6, 298 K). The arrows highlight the chemical shift perturbation of selected residues. (B) Overlay of 1H-15N HSQC spectra highlighting the chemical shift perturbations of selected CTD (100 μM) residues upon addition of small aliquots for s2m-CoV-2 (25 mM Na Phos, 50 mM NaCl pH 6, 303 K). (C) Depiction of the cross-linking counts obtained by CLIR-MS for each amino acid residue of CTD upon addition of s2m-CoV-2, s2m-CoV-1 without Mg2+ and s2m-CoV-1 in the presence of Mg2+. (D) Plot of combined 1H and 15N chemical shift perturbation induced by s2m-CoV-2 to the CTD amino acid residues at 0.03:1 ratio before complete signal broadening. (E) HADDOCK model 1 of the interaction between CTD and s2m-CoV-2 with highlighted experimental restraints used and focus on key interaction sites.