Figure 2.

CRISPR/Cas9-CtIP-dnRNF168 promotes gene conversion using ssODN as repair template. (A) Co-delivery of the CRISPR/Cas9 together with single-stranded oligodeoxynucleotides (ssODN) harboring the nucleotide changes indicated in red (194 G > C and 196 C > T) results in BFP to GFP conversion (upper panel). Shown are exemplary plots that illustrate the extent of GFP+ cells resulting from gene conversion triggered by the indicated nucleases (lower panel). (B) The bar graph indicates the extent of BFP-to-GFP gene conversion measured via flow cytometry as percentage of GFP + cells. Experiments were performed in duplicate and pooled data are shown as black dots. Statistically significant differences, as compared to the unmodified Cas9 nuclease, are indicated with asterisks and correspond to p-values calculated with ANOVA (**P< 0.01). Error bars indicate SEM.