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. 2023 Mar 2;51(9):4284–4301. doi: 10.1093/nar/gkad120

Figure 1.

Figure 1.

BACH1 highly correlates with VSMC identity. (A) Viewpoint plot of peak-promoter co-accessibility and the snATAC-Seq tracks centered at the CAD/MI SNP rs73193808 in VSMCs of human atherosclerotic lesion. (B) Motif enrichment z-scores were computed by chromVAR and enrichment z-scores for BACH1, KLF4, and SRF motifs in VSMCs of human atherosclerotic lesion projected onto UMAP coordinates. (C) Heatmap of selected TF activities inferred with DoRothEA from gene expression data generated via human atherosclerotic lesion 10X scRNA-Seq. (D) Heatmap of the expression of VSMC marker genes (ACTA2, CNN1), BACH1, and KLF4 along pseudotime in VSMCs of human atherosclerotic lesion. Contractile SMCs were located at origin of the trajectory (right) and Synthetic SMCs were located towards the termini of the trajectory (left). (E) The coronary artery sections from patients who had CAD or who died from non-cardiovascular diseases were immunostained for BACH1 (brown) and TAGLN (red). Representative images shown. Scale bar: 50μm. Quantitative and statistical analysis are shown in Online Figure I A (control n = 6, CAD n = 7, data are mean ± SD, unpaired towo-tail t-test).