Figure 4.

Cryo-EM 3D variability analysis of Pepper and Broccoli aptamers. (A) Representative structures are shown for the principal component analysis (PC0, PC1 and PC2). Arrows indicate the most prominent movements. Gaussian distribution of particles along two principal reaction coordinates (PC1, PC2) determined by 3DVA (right). (B) Intermediate reconstructions using particle subsets from the extremes of PC0 and PC1 show the positional variability of broccoli and pepper aptamers. (C) HBC ligands from pepper were aligned while maintaining the spatial relationship with the DFHBI-1T ligand from Broccoli from the extrema reconstructions. Distances from HBC to the center of each DFHBI-1T were measured as well as the furthest distance between DFHBI-1T fluorophores.