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. 2023 Mar 23;51(9):4536–4554. doi: 10.1093/nar/gkad193

Table 3.

Effects of CplR functionality on C. perfringens resistance to PLSA and macrolide antibiotics

MIC (μg/ml)
C. perfringens strain erm A201A Hygromycin A Retapamulin Lincomycin Clindamycin Iboxamycin Virginiamycin M1 Erythromycin
wt/pJIR750 - 4 4 0.8 4 2 0.0156 3.2 1
ΔcplR/pJIR750 - 4 4 0.025 0.25 <0.0625 0.0078 1.6 1
ΔcplR/pJIR750-cplR - 8 8 12.8 32 16 0.125 3.2 1

The MIC test for C. perfringens was carried out in GAM media and the growth inhibition was scored after 24 h of anaerobic incubation at 37°C. In the case of plasmid-harboring strains GAM media was attentionally supplemented with 10 μg/ml chloramphenicol to maintain the plasmids. Expression of the CplR ARE-ABCF was driven by the native promotor.