Table 3.
Effects of CplR functionality on C. perfringens resistance to PLSA and macrolide antibiotics
MIC (μg/ml) | |||||||||
---|---|---|---|---|---|---|---|---|---|
C. perfringens strain | erm | A201A | Hygromycin A | Retapamulin | Lincomycin | Clindamycin | Iboxamycin | Virginiamycin M1 | Erythromycin |
wt/pJIR750 | - | 4 | 4 | 0.8 | 4 | 2 | 0.0156 | 3.2 | 1 |
ΔcplR/pJIR750 | - | 4 | 4 | 0.025 | 0.25 | <0.0625 | 0.0078 | 1.6 | 1 |
ΔcplR/pJIR750-cplR | - | 8 | 8 | 12.8 | 32 | 16 | 0.125 | 3.2 | 1 |
The MIC test for C. perfringens was carried out in GAM media and the growth inhibition was scored after 24 h of anaerobic incubation at 37°C. In the case of plasmid-harboring strains GAM media was attentionally supplemented with 10 μg/ml chloramphenicol to maintain the plasmids. Expression of the CplR ARE-ABCF was driven by the native promotor.