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. 2023 Mar 17;51(9):4341–4362. doi: 10.1093/nar/gkad172

Figure 1.

Figure 1.

CRISPR-Cas9 dropout screen identifies MEPCE as a potential vulnerability in BRCA1-mutated breast cancers. (A) Schematic of CRISPR screening workflow. (B) Venn diagram representation of high-confidence negatively selected genes in all cell lines. (FDR < 0.1). 2 replicates for each time point were used. (C, D) Dot plot representing gene essentiality in BRCA1 WT cell line compared to BRCA1-mutated cell lines. C:MDA-MB-436 vs. MDA-MB-231. D:SUM149PT vs. MDA-MB-231. Vertical and horizontal dotted lines show 0.1 FDR cut-offs. Control sgRNA construct (sgLacZ) and essential genes PSMD1 and PSMB2 are highlighted. Only MEPCE demonstrates differential essentiality. (E) Scatter plot demonstrating negative correlation between the expression levels of MEPCE and BRCA1 in all TCGA breast cancer samples.