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. 2023 Apr 18;51(9):4674–4690. doi: 10.1093/nar/gkad273

Figure 1.

Figure 1.

Design of molecular components and experimental setup used to study how transcription factor dynamics are decoded by mammalian gene regulatory elements. (A) Schematic representation of the expected behavior of the synthetic transcription factor (synTF) in darkness (left) and during blue light illumination (right). Blue light leads to the exposure of the nuclear localization signal (NLS), which, in turn, leads to the nuclear import of synTF. LexA, full-length bacterial repressor used as DNA binding domain (DBD) within synTF. VP48, 3x residues 436–47 of the VP16 transactivation domain (TAD). NES, nuclear export signal. (B) Schematic representation of the illumination schemes used in this study to achieve similar cumulative TF levels (area under the curve) for the different TF dynamics. (C) Schematic overview of the experimental setup. (D) Schematic representation of the reporter libraries used in this study.