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. 2023 Apr 18;51(9):4674–4690. doi: 10.1093/nar/gkad273

Figure 2.

Figure 2.

A promoter with strong RE and TATA box does not distinguish TF dynamics. (A-C) Quantification of synTF nuclear translocation (A), mean reporter nascent RNA (B) and protein (C) levels over time for the indicated TF dynamics in combination with promoter p1. Light blue shadowing, blue light illumination phase. Together with the experimental data (black dots), fitted (violet line; for sustained dynamics) and simulated (pink line; for both pulsatile dynamics) values are shown. The mathematical model used is shown in Figure 4 and the equations are described in the Supplememtary Text. (D, G, J) Quantification of mean nascent RNA per cell per minute (calculated averaging over the entire blue light illumination phase) for the indicated synTF dynamics in combination with promoters p1 (D), p2 (G) and p3 (J). (E, H, K) Quantification of reporter protein expression levels at the end of the experiment for the indicated synTF dynamics in combination with promoters p1 (E), p2 (H) and p3 (K). (F, I, L) Quantification of reporter protein expression levels at the end of the experiment for high (dark colors) or low (light colors) synTF amplitudes for the indicated synTF dynamics in combination with promoters p1 (F), p2 (I) and p3 (L). (E, F, H, I, K, L) P-values were calculated with the Welch's t-test. ns, non signficant (P > 0.05). (E) *P-value = 0.0244; **P-value = 0.003. (L) *P-value (from left to right) = 0.02552, 0.01285, 0.03517. Data represent mean ± s.e.m. of at least n = 20 individual cells, imaged on at least n = 3 biologically independent experiments.