Table 2.

Parameters for ΔG°_{37, NN, bulk}, ΔG°_{37, NN, [122 mM Na}⁺_], ΔG°_{37, NN, ev} and ΔG°_{37, NN, wa} in 40 wt% PEG200 along with prefactors (m_cs) for different cosolutes^a

Sequence	ΔG°37, NN, bulk (kcal mol−1)	ΔG°37, NN, [122 mM Na+]b (kcal mol−1)	ΔG°37, NN, ev [40 wt% PEG200]c (kcal mol−1)	ΔG°37, NN, wa [40 wt% PEG200] (kcal mol−1)	m PEG/2-ME/1,2 DME (kcal mol−1)	m EG/Gly/1,3 PDO (kcal mol−1)
r(AA/UU)	−0.70	−0.00	−0.22	0.35	7.1	2.9
r(AU/UA)	−0.22	−0.30	−0.22	0.19	3.9	1.6
r(UA/AU)	−1.22	−0.08	−0.22	0.19	3.9	1.6
r(CA/GU)	−1.69	−0.09	−0.22	−0.14	−2.9	−1.2
r(GU/CA)	−1.73	−0.41	−0.22	0.56	11.4	4.7
r(CU/GA)	−1.52	−0.28	−0.22	0.25	5.1	2.1
r(GA/CU)	−2.06	−0.16	−0.22	0.20	4.1	1.7
r(CG/GC)	−2.00	−0.18	−0.22	0.24	4.9	2.0
r(GC/CG)	−2.72	−0.58	−0.22	0.45	9.2	3.8
r(GG/CC)	−2.92	−0.18	−0.22	0.43	8.8	3.6
Initiation	4.09	0.0d	−0.22	1.63	33.3	13.7
Per terminal AU	0.45	0.0d	NAe	0.40	8.2	3.4

^aCorrection factor for self-complementary sequences is the same (0.43 kcal mol⁻¹) for all cosolutes because it is independent of the solution condition. ^bΔG°_{37, NN, cat} for any concentration of Na⁺ can be obtained from the Na⁺ dependency of individual NN pairs, as mentioned in Supplementary Figure S3. ^cAveraged over all 45 sequences used in this study. ^dTerminal pairs were assumed to be independent of Na⁺ concentration following the report by Huguet et al. (47) and thus, cation-induced stabilization was not added to terminal pairs. ^eExcluded volume effect for terminal AU pairs was not considered to avoid overestimation of the effect, as it was already considered for initiation.