Table 2.
Experimentally validated stable pG4-BS located in selected cancer-associated genes
| Gene symbol | Entrez ID | Gene description | Chromosome | pG4-BS start | pG4-BS end | pG4-BS strand | Gene orientation | Sequence ID | Sequence with paired nucleotides on flanks | Number of intact G-stems | Number of bulges | Inserted nucleotides | Number of cytosines (C) | NMR spectrum model | CD Spectrum: antiparallel = 0, parallel = 1 | UV melting temperature (°C) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTN4 | 81 | actinin alpha 4 | chr19 | 38648874 | 38648892 | + | Sense | BS31 | GA-GATCGG-T-GGG-A-GGG-A-GGG-TG | 3 | 1 | ATC | 1 | Single G4 | 1 | 51 |
| ANPEP | 290 | alanyl aminopeptidase, membrane | chr15 | 89814896 | 89814915 | - | Sense | BS36 | TC-GGG-T-GGAG-TA-GTGG-TC-GGG-TC | 2 | 2 | A,T | 1 | Multiple G4 | 0 | – |
| BLZF1 | 8548 | basic leucine zipper nuclear factor 1 | chr1 | 169368218 | 169368236 | + | Sense | BS45 | AT-GCGG-A-GGG-ACT-GGG-C-GGG-TC | 3 | 1 | C | 2 | Single G4 | 1 | 62 |
| CACNA2D2 | 9254 | calcium voltage-gated channel auxiliary subunit alpha2/delta 2 | chr3 | 50503703 | 50503720 | - | Sense | BS40 | AA-GAGAG-A-GGG-A-GGG-A-GGG-AG | 3 | 2 | A,A | 0 | Single G4 | – | <40 |
| CASP8 | 841 | caspase 8 | chr2 | 201257751 | 201257767 | + | Sense | BS20 | GC-GGG-A-GGG-A-GGAG-A-GGG-CT | 3 | 1 | A | 0 | Major + minor G4 | 1 | 55 |
| CBX4 | 8535 | chromobox 4 | chr17 | 79840014 | 79840034 | + | Antisense | BS47 | GC-GAGG-A-GGG-AAA-GGG-ACA-GGG-CG | 3 | 1 | A | 0 | Major + minor G4 | 0 | 38 |
| CCR7 | 1236 | C–C motif chemokine receptor 7 | chr17 | 40563535 | 40563551 | - | Sense | BS17 | GT-GGG-T-GAGG-A-GGG-A-GGG-TT | 3 | 1 | A | 0 | Major + minor G4 | 1 | 63 |
| DNAJB1 | 3337 | DnaJ heat shock protein family (Hsp40) member B1 | chr19 | 14518583 | 14518598 | - | Sense | BS2 | TT-GGG-C-GGAG-C-GGG-A-GGG | 3 | 1 | A | 2 | Major + minor G4 | – | 66 |
| E2F8 | 79733 | E2F transcription factor 8 | chr11 | 19241765 | 19241782 | - | Sense | BS33 | TA-GAGG-C-GGG-A-GTGG-A-GGG-CG | 2 | 2 | A,T | 0 | Major + minor G4 | 1 | 50 |
| EGR1 | 1958 | early growth response 1 | chr5 | 138464809 | 138464825 | + | Sense | BS15 | CT-GAGG-T-GGG-C-GGG-C-GGG-CC | 3 | 1 | A | 2 | Single G4 | 1 | 79 |
| EZR | 7430 | ezrin | chr6 | 158818194 | 158818211 | - | Sense | BS14 | AC-GAGG-CA-GGG-C-GGG-C-GGG-CG | 3 | 1 | A | 3 | Single G4 | 1 | 72 |
| GDF15 | 9518 | growth differentiation factor 15 | chr19 | 18387011 | 18387031 | + | Sense | BS48 | AT-GAGG-TCT-GGG-CT-GTGG-T-GGG-AC | 2 | 2 | A,T | 1 | Multiple G4 | 0 | – |
| LMO4 | 8543 | LIM domain only 4 | chr1 | 87329129 | 87329146 | - | Antisense | BS30 | GA-GCTGG-A-GGG-A-GGG-A-GGG-AG | 3 | 1 | CT | 1 | Multiple G4 | 1 | – |
| MECOM | 2122 | MDS1 and EVI1 complex locus | chr3 | 169321683 | 169321699 | + | Antisense | BS16 | TT-GTGG-A-GGG-A-GGG-T-GGG-AC | 3 | 1 | T | 0 | Single G4 | 1 | 76 |
| MECOM | 2122 | MDS1 and EVI1 complex locus | chr3 | 169663029 | 169663045 | - | Sense | BS28 | AG-GGG-T-GGG-A-GGG-A-GGAG-AG | 3 | 1 | A | 0 | Multiple G4 | 1 | – |
| MYCN | 4613 | MYCN proto-oncogene, bHLH transcription factor | chr2 | 15943554 | 15943572 | - | Antisense | BS32 | CT-GAATGG-A-GGG-A-GGG-A-GGG-TG | 3 | 1 | AAT | 0 | Single G4 | 1 | 43 |
| NF1 | 4763 | neurofibromin 1 | chr17 | 31095380 | 31095397 | + | Sense | BS25 | CC-GTGG-C-GGG-C-GGG-A-GGTG-GG | 2 | 2 | T,T | 2 | multiple G4 | 1 | – |
| NF1 | 4763 | neurofibromin 1 | chr17 | 31095380 | 31095399 | + | Sense | BS26 | CC-GTGG-C-GGG-C-GGG-AGGT-GGG-AG | 3 | 1 | T | 2 | Multiple G4 | 1 | – |
| NRP2 | 8828 | neuropilin 2 | chr2 | 205685856 | 205685873 | + | Sense | BS35 | GA-GGG-C-GGAG-A-GAGG-C-GGG-AA | 2 | 2 | A,A | 2 | Single G4 | 1 | 49 |
| NTNG1 | 22854 | Netrin G1, transcript variant 11 | ch1 | 107141271 | 107141287 | - | Antisense | BS9 | TT-GGG-C-GGAG-C-GGG-AG-GGG | 3 | 1 | A | 2 | Major + minor G4 | 1 | – |
| PTGFRN | 5738 | prostaglandin F2 receptor inhibitor | chr1 | 116909887 | 116909902 | + | Sense | BS1 | TT-GGG-A-GGCG-C-GGG-C-GGG | 3 | 1 | C | 3 | Major + minor G4 | – | 69 |
| PVT1 | 5820 | Pvt1 oncogene | chr8 | 127795677 | 127795693 | + | Sense | BS51 | GT-GTGG-T-GGG-T-GGG-C-GGG-GG | 3 | 1 | T | 1 | Major + minor G4 | – | >80 |
| RARA | 5914 | retinoic acid receptor alpha | chr17 | 40341607 | 40341624 | + | Sense | BS18 | AC-GGG-CA-GCGG-T-GGG-T-GGG-TC | 3 | 1 | C | 2 | Single G4 | 1 | 70 |
| RARA | 5914 | retinoic acid receptor alpha | chr17 | 40350488 | 40350505 | + | Sense | BS24 | CT-GATGG-A-GGG-A-GGG-A-GGG-AA | 3 | 1 | AT | 0 | Major + minor G4 | 1 | 47 |
| RASSF1 | 11186 | Ras association domain family member 1 | chr3 | 50337643 | 50337660 | + | Antisense | BS22 | GA-GGG-C-GGG-AA-GGTG-C-GGG-AA | 3 | 1 | T | 2 | Single G4 | 1 | 63 |
| RGL2 | 5863 | ral guanine nucleotide dissociation stimulator like 2 | chr6 | 33299346 | 33299364 | - | Sense | BS19 | GT-GGG-TT-GTGG-TA-GGG-A-GGG-TA | 3 | 1 | T | 0 | Major + minor G4 | 0 | 61 |
| RPN1 | 6484 | ribophorin I | chr3 | 128650848 | 128650864 | - | Sense | BS13 | CA-GTGG-C-GGG-A-GGG-C-GGG-CT | 3 | 1 | T | 2 | Single G4 | 1 | 78 |
| VWF | 7450 | von Willebrand factor | chr12 | 6124638 | 6124656 | - | Sense | BS46 | TT-GTGG-T-GGG-AAA-GGG-A-GGG-TG | 3 | 1 | T | 0 | Major + minor G4 | 1 | 56 |