Table 5.
Top 10 overall ranked vaccine candidates specific to cell-mediated immunity.
| RH88 ID | Name | Len | Toxicity | Auto-immunity | No. of Strains | Tz rank | Bz rank | Sp rank | Overall rank |
|---|---|---|---|---|---|---|---|---|---|
| TGRH88_081130 | dense granule protein GRA1 | 190 | Non-Toxin | Safe | 15 | 8 | 24 | 1 | 1 |
| TGRH88_020190 | rhoptry protein ROP7 | 575 | Non-Toxin | Safe | 14 | 39 | 2 | 12 | 2 |
| TGRH88_046790 | dense granule protein GRA3 | 222 | Non-Toxin | Safe | 15 | 5 | 59 | 2 | 3 |
| TGRH88_050950 | rhoptry neck protein RON5 | 1702 | Non-Toxin | Safe | 15 | 20 | 35 | 17 | 4 |
| TGRH88_051090 | chaperonin protein BiP | 668 | Non-Toxin | Potential | 15 | 23 | 17 | 33 | 5 |
| TGRH88_062360 | ribosomal protein RPS9 | 188 | Non-Toxin | Potential | 15 | 43 | 15 | 25 | 6 |
| TGRH88_077370 | unspecified product | 1049 | Non-Toxin | Safe | 15 | 41 | 34 | 14 | 7 |
| TGRH88_043710 | rhoptry neck protein RON3 | 1958 | Non-Toxin | Safe | 15 | 18 | 41 | 43 | 8 |
| TGRH88_033050 | poly(ADP-ribose) glycohydrolase | 553 | Non-Toxin | Safe | 15 | 74 | 49 | 4 | 9 |
| TGRH88_001930 | unspecified product | 519 | Non-Toxin | Safe | 15 | 60 | 43 | 44 | 10 |
Key to columns: Len = protein length; Toxicity = a ‘Toxin’ or ‘Non-Toxin’ classification indicating a protein’s potential toxicity as predicted by the program ToxinPred2; Autoimmunity = a ‘Safe’ or ‘Potential’ classification indicating the potential of the protein to trigger an autoimmune response in humans (based on sequence similarity between RH-88 Toxplasma gondii and humans as calculated by BLASTP); No. of Strains = number of different strains that have a protein with a sequence similarity > 90% to the RH88 ID protein sequence; Tz_rank, Bz_rank, and Sp_rank contain the vaccine candidacy rank that is specific to the infection stage of tachyzoites (Tz), bradyzoites (Bz), and sporozoites (Sp), respectively; Overall rank = a rank taking into account Tz_rank + Bz_rank + Sp_rank.