Table 6.
Top 10 overall ranked vaccine candidates specific to humoral immunity.
| RH88 ID | Name | Len | Toxicity | Auto-immunity | No. of Strains | Under PS | Tz rank | Bz rank | Sp rank | Overall rank |
|---|---|---|---|---|---|---|---|---|---|---|
| TGRH88_038710 | Dense granule protein GRA6 | 201 | Non-Toxin | Safe | 15 | 7 | 3 | 4 | 1 | 1 |
| TGRH88_025740 | Dense granule protein GRA5 | 120 | Non-Toxin | Safe | 15 | 1 | 1 | 6 | 4 | 2 |
| TGRH88_022490 | Putative myosin heavy chain | 1124 | Non-Toxin | Safe | 15 | 0 | 4 | 2 | 10 | 3 |
| TGRH88_011090 | 3′5'-cyclic nucleotide phosphodiesterase domain-containing protein | 1065 | Non-Toxin | Safe | 15 | 0 | 2 | 8 | 9 | 4 |
| TGRH88_022410 | Protease inhibitor PI2 | 318 | Toxin | Safe | 15 | 0 | 13 | 5 | 2 | 5 |
| TGRH88_068970 | Unspecified product | 131 | Non-Toxin | Safe | 15 | 15 | 9 | 20 | 3 | 6 |
| TGRH88_037170 | Microneme protein MIC2 | 769 | Toxin | Safe | 15 | 0 | 14 | 12 | 6 | 7 |
| TGRH88_066240 | Microneme protein MIC5 | 181 | Non-Toxin | Safe | 15 | 0 | 12 | 11 | 11 | 8 |
| TGRH88_033800 | Cyclophilin precursor | 348 | Non-Toxin | Safe | 15 | 0 | 5 | 19 | 18 | 9 |
| TGRH88_015150 | Unspecified product | 312 | Non-Toxin | Safe | 15 | 1 | 18 | 15 | 13 | 10 |
Key to columns: Len = protein length; Toxicity = a ‘Toxin’ or ‘Non-Toxin’ classification indicating a protein’s potential toxicity as predicted by the program ToxinPred2; Autoimmunity = a ‘Safe’ or ‘Potential’ classification indicating the potential of the protein to trigger an autoimmune response in humans (based on sequence similarity between RH-88 Toxplasma gondii and humans as calculated by BLASTP); No. of Strains = number of different strains that have a protein with a sequence similarity > 90% to the RH88 ID protein sequence; Under PS = number of positive selection sites as predicted by Bayes Empirical Bayes (BEB) analysis within CODEML; Tz_rank, Bz_rank, and Sp_rank contain the vaccine candidacy rank that is specific to the infection stage of tachyzoites (Tz), bradyzoites (Bz), and sporozoites (Sp), respectively; Overall rank = a rank taking into account Tz_rank + Bz_rank + Sp_rank.