FIG. 2.

Assembly and budding profile of PPXY mutants. BHK-21 cells were infected with either the WT or mutant viruses at an MOI of 10. Cells were labeled at 7 h p.i. with [³⁵S]methionine. At 15 h p.i., viruses were harvested from the supernatants by centrifugation and analyzed by SDS-PAGE followed by autoradiography. The positions of the five VSV proteins are indicated on the right. The mutated residue(s) in the PPPY sequence of each mutant is underlined. The percent yield of virus in this experiment is calculated based on the quantitation of N protein using ImageQuant software (Molecular Dynamics).