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Comparison of the plaque sizes of WT and PPXY mutants in BHK-21 cells. (A) A standard plaque assay was performed for each virus on BHK-21 cells. At 13 h p.i., the sizes of 25 individual plaques were determined for each virus. The mean plaque size for each virus is shown. The residue(s) changed in each mutant is underlined. (B) Fluorescence micrograph of GFP-expressing cells comparing the plaque sizes of WT and the PPPA mutant (magnification, ×7.5).
