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Growth kinetics of PPXY mutants in BHK-21 cells. BHK-21 cells were infected with either the WT or the mutant viruses at an MOI of 10. At various times p.i., aliquots of supernatant were taken to determine the titer by a plaque assay on BHK cells. The kinetics for the P and Y mutants were determined simultaneously under similar conditions, but they were plotted individually to show the growth difference during early times of infection.
