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. 2023 May 22;14(1):115–127. doi: 10.1016/j.jpha.2023.05.011

Fig. 5.

Fig. 5

Anti-inflammatory effects of licorice-saponin A3 (A3) in lipopolysaccharide (LPS)-induced mice acute lung injury (ALI) mice model. (A) Experimental scheme to construct LPS-induced ALI mice model. (B) Representative histopathology of lung sections of ALI mice with or without A3 administration. Control group: healthy mice treated with saline solution; Model group: LPS-induced ALI mice without A3 administration; A3 groups: LPS-induced ALI mice with A3 administration in a dose of 10 or 20 mg/kg, i. g., respectively, n = 4. The scale bars represent 500 μm for × 5 magnification images and 100 μm for × 20 magnification images. (C) Pathology evaluation for the therapeutic effects of A3 in LPS-induced ALI as shown in Fig. 5B, n = 4, ∗∗P < 0.01, ∗∗∗P < 0.001. (D) Tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β and IL-7 of blood samples were measured by mouse enzyme linked immunosorbent assay (ELISA) kit (MEIMIAN, www.mmbio.cn). ∗P < 0.05, ∗∗P < 0.01 compared with the control group, n = 4. (E) Representative immunohistochemistry (IHC) of lung sections of LPS-induced ALI mice with or without A3 administration for TNF-α, IL-6, IL-1β and IL-7 analysis.