FIG. 7.

Decreased viral infectivity for newly infected P2-ACH.p12 cells in primary lymphocytes. Quiescent and activated PBMC were cocultured with PBMC populations newly infected with ACH or ACH.p12 (P2-ACH and P2-ACH.p12) for 7 days in 15% cRPMI in the absence of stimuli, such as IL-2 or PHA. After coculture, cells were washed and reseeded in fresh medium including hIL-2 (10 U/ml). Supernatants were assayed for viral p19 antigen production by ELISA for 28 days postwash. Data points are the means of triplicate samples ± SEM and represent two independent experiments. A significantly reduced infectivity was observed for P2-ACH.p12 in quiescent and activated PBMC target cells (Student's t test, P < 0.01).