TABLE 1.

Amino acid changes at positions 72, 73, 74, and 204 selected in vivo^a

^aAmino acid sequences at positions 72, 73, 74, and 204 in Nef, derived from DNA population sequencing, are shown for each of the macaques at the indicated weeks postinfection (w). DNA was obtained from bulk cocultivations of CEMx174 cells with rPBMC collected at the indicated time points or from lymph node biopsy (∗). Similar results were obtained with DNA isolated directly from rPBMC and with genomic viral RNA amplified by reverse transcription-PCR. The results were also confirmed by sequence analysis of 131 single PCR clones. The percentage of sequences that encode a given residue is indicated as a subscript. Dashes indicate identity to the sequence of the input mutant. For animal Mm8155, the last sequence was obtained at the time of death (21 wpi).