Skip to main content
. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Nat Immunol. 2022 Nov 21;23(12):1714–1725. doi: 10.1038/s41590-022-01349-1

Figure 1. MAIT cells are present in the meninges.

Figure 1.

a, Representative flow cytometry plots of MAIT cells in the meninges and choroid plexus (CP) of C57BL/6 mice of different ages. Plots were pre-gated on CD45+CD11b−B220−NK1.1− Thy1.2+ cells. Gating strategy is shown in Supplementary Figure 1a. b, Numbers of MAIT cells at different regions of the brain in mice of different ages. 6w, 6 weeks; 7m, 7 months; 18m, 18 months. c, Representative flow cytometry plots of MAIT cells in the meninges of 7-month-old Mr1+/+ and Mr1−/− mice. d, Numbers of MAIT cells in the meninges of 7-month-old Mr1+/+ and Mr1−/− mice. e, Representative histogram plots depict expression of the indicated genes in meningeal MAIT cells (CD3+ MR1-tetramer+) and non-MAIT T cells (CD3+ MR1-tetramer−). f, Representative flow cytometry plots of MAIT cells in the dura/arachnoid meningeal tissue isolated from the inner calvaria, or in the leptomeninges. g, Frequencies of MAIT cells in the dura/arachnoid meningeal tissue isolated from the inner calvaria, or in the leptomeninges. Error bars = Mean ± SE. Data are from 6 mice per group, pooled from 2 independent experiments (a,b), or from 10 mice per group, pooled from 2 independent experiments (c,d), or from 4 mice pooled per sample per experiment, representative of 3 independent experiment (e), or from 6 samples per group, 2 mice pooled per sample, pooled from 2 independent experiments (g). *P < 0.05, **P < 0.01 using two-sided ANOVA (b) or two-sided Student’s t-test (d, g); exact P values are provided in the source data.