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. 2000 Nov;74(21):9845–9857. doi: 10.1128/jvi.74.21.9845-9857.2000

FIG. 2.

FIG. 2

FIG. 2

Structural analysis of recombinant viruses RVwt-xs, RVdlNF-Y-xs, RVdlElk-1/IE86-xs, RVwt-as, and RVdlIE86-as. (A) Maps of RVUL4CATgpt, RVwt-xs, RVdlNF-Y-xs, RVdlElk-1/IE86-xs, RVwt-as, and RVdlIE86-as. The sizes of the DNA fragments resulting from HindIII, HaeII, or SfiI restriction endonuclease digestion are indicated in base pairs. The genes involved in homologous recombination in shuttle vectors are shown by shaded boxes. A, S, H, and X stand for the restriction endonuclease sites AvrII, SacI, HindIII, and XbaI, respectively. (B to F) Individual autoradiograms of Southern blots to identify the recombinant viruses using either 32P-labeled XA probe (B to D) or HindIII X probe (E and F). Lanes containing viral DNA fragments from different recombinant viruses were spliced together from the same gel. Shuttle vectors pwt-xs, pdlNF-Y-xs, pdlElk-1/IE86-xs, and pdlIE86-as were used as positive controls.